I-309 qPCR Primer Pairs, Human

Cat: HP100145
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I-309 qPCR Primer Pairs, Human 基本信息
Target Details
种属:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
运输:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
I-309 qPCR Primer Pairs, Human Alternative Names
I-309 qPCR Primer Pairs, Human;P500 qPCR Primer Pairs, Human;SCYA1 qPCR Primer Pairs, Human;SISe qPCR Primer Pairs, Human;TCA3 qPCR Primer Pairs, Human
I-309 Background Information

CCL1 or chemokine (C-C motif) ligand 1, also known as I-309 or TCA-3, is a member of the chemokine (C-C motif) ligand family. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands (CCL)-1 to 28. I-309/CCL1/TCA-3 interacts with the G protein-linked transmembrane chemokine receptors CCR8, and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that I-309/CCL1/TCA-3 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, I-309/CCL1/TCA-3 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. I-309/CCL1/TCA-3 induce significant levels of LTC4 from elicited eosinophils.

Full Name
chemokine (C-C motif) ligand 1
References
  • Miller MD, et al. (1992) The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci. 89 (7): 2950-4.
  • Roos RS, et al. (1997) Identification of CCR8, the receptor for the human CC chemokine I-309. J Biol Chem. 272 (28): 17251-4.
  • Oliveira SH, et al. (2002) Increased responsiveness of murine eosinophils to MIP-1beta (CCL4) and TCA-3 (CCL1) is mediated by their specific receptors, CCR5 and CCR8. J Leukoc Biol. 71(6): 1019-25.
  • I-309 qPCR Primer Pairs, Mouse, Related Products

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    I-309 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    I-309 cDNA ORF Clone in Cloning Vector, Human HG10057-M
    I-309 cDNA ORF Clone, Human, untagged HG10057-UT
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    I-309 cDNA ORF Clone, Human, C-GFPSpark® tag HG10057-ACG
    I-309 cDNA ORF Clone, Human, C-OFPSpark® tag HG10057-ACR
    I-309 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10057-CF
    I-309 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10057-NF
    I-309 cDNA ORF Clone, Human, C-His tag HG10057-CH
    I-309 cDNA ORF Clone, Human, N-His tag HG10057-NH
    I-309 cDNA ORF Clone, Human, C-HA tag HG10057-CY
    I-309 cDNA ORF Clone, Human, N-HA tag HG10057-NY
    I-309 cDNA ORF Clone, Human, C-Myc tag HG10057-CM
    I-309 cDNA ORF Clone, Human, N-Myc tag HG10057-NM
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