PDGFRB cDNA ORF Clone, Human, N-HA tag

Cat: HG10514-NY
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PDGFRB cDNA ORF Clone, Human, N-HA tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
3321 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human platelet-derived growth factor receptor, beta polypeptide with N terminal HA tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-N-HA
限制性酶切位点
KpnI + XbaI (6kb + 3.36kb)
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
PDGFRB cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PDGFRB cDNA ORF Clone, Human, N-HA tag Validated Images
Rhesus CD3d/CD3 delta Gene Plasmid Map 5616
Human PDGFRB/CD140b Gene Expression validated Image 16814
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-HA Tag monoclonal antibody (100028-MM10) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
PDGFRB cDNA ORF Clone, Human, N-HA tag Alternative Names
CD140B cDNA ORF Clone, Human;IBGC4 cDNA ORF Clone, Human;IMF1 cDNA ORF Clone, Human;JTK12 cDNA ORF Clone, Human;KOGS cDNA ORF Clone, Human;PDGFR cDNA ORF Clone, Human;PDGFR-1 cDNA ORF Clone, Human;PDGFR1 cDNA ORF Clone, Human;PENTT cDNA ORF Clone, Human
PDGFRB Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
platelet-derived growth factor receptor, beta polypeptide
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Suzuki S, et al. (2010) Clinicopathological significance of platelet-derived growth factor (PDGF)-B and vascular endothelial growth factor-A expression, PDGF receptor- phosphorylation, and microvessel density in gastric cancer. BMC cancer. 10: 659.
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