CD30 / TNFRSF8 ELISA Kit, Human

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CD30 / TNFRSF8 ELISA Kit, Human: 产品信息

产品名称
CD30 / TNFRSF8 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Lysate
灵敏度
11.8 pg/mL
线性范围
23.44-1500 pg/mL
特异性
Recognizes both recombinant and natural Human CD30 / TNFRSF8
干扰
Preparations of TNFSF8 and BCL6 at 15 ng/mL in a mid-range recombinant human CHL1 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.23 0.47 0.94
SD 0.01 0.02 0.03
CV(%) 5.7% 4.6% 3.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.25 0.48 0.95
SD 0.02 0.03 0.07
CV(%) 9.4% 5.6% 6.9%
回收率
The recovery of Human TNFRSF8 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
86
Range
82-90%
线性关系
Supernatant
  Recovery of detected
1:2 90%
1:4 93%
1:8 105%
1:16 129%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD30 / TNFRSF8 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD30 / TNFRSF8 protein in Lysate . It contains recombinant Human CD30 / TNFRSF8, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD30 / TNFRSF8 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human TNFRSF8. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD30 / TNFRSF8 ELISA Kit, Human: 别称

CD30 ELISA Kit, Human; D1S166E ELISA Kit, Human; Ki-1 ELISA Kit, Human

CD30 / TNFRSF8 背景信息

CD3, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor (TNFR) superfamily. CD3 protein is expressed by activated, but not resting, T and B cells. CD3 can regulate proliferation of lymphocytes and may also play an important role in human immunodeficiency virus replication. As a regulator of apoptosis, CD3 protein induces cell death or proliferation, depending on the cell type, and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. CD3 protein expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs), and CD3 is also linked to leukocytes in patients with chronic inflammatory diseases, including lupus erythematosus, asthma, rheumatoid arthritis and atopic dermatitis (AD).
全称
tumor necrosis factor receptor superfamily, member 8
研究领域
参考文献
  • Rossi FM, et al. (2001) CD30L up-regulates CD30 and IL-4 expression by T cells. FEBS Lett. 508(3): 418-22.
  • Trovato M, et al. (2001) Expression of CD30 ligand and CD30 receptor in normal thyroid and benign and malignant thyroid nodules. Thyroid. 11(7): 621-8.
  • Ekstrom ES, et al. (2001) Presence of CD30(+) and CD30L(+) cells in human placenta and soluble CD30 levels in cord blood are independent of maternal atopy. Placenta. 22(4): 372-9.
  • Tang C, et al. (2008) A novel role of CD30L/CD30 signaling by T-T cell interaction in Th1 response against mycobacterial infection. J Immunol. 181(9): 6316-27.
  • Sun X, et al. (2008) A critical role of CD30 ligand/CD30 in controlling inflammatory bowel diseases in mice. Gastroenterology. 134(2): 447-58.
  • Oflazoglu E, et al. (2009) Targeting CD30/CD30L in Oncology and Autoimmune and Inflammatory Diseases.Adv Exp Med Biol. 647: 174-85.
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