|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13365-ACG|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13365-ACR|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13365-CF|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13365-CH|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13365-CM|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13365-CY|
|人 CLM-9 / TREM4 基因ORF全长cDNA(克隆载体)||HG13365-G|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13365-NF|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13365-NH|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13365-NM|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13365-NY|
|人 CLM-9 / TREM4 基因ORF全长cDNA克隆(表达载体)||HG13365-UT|
CLM-9, also known as TREM4, is a receptor which belongs to the TREM family. The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. CLM-9 may mediate L-selectin-dependent lymphocyte rollings. It binds SELL in a calcium dependent manner. CLM-9 also binds lymphocyte which suggests that it functions in lymphocyte adhesion. The major CLM-9 transcript is expressed highly in human heart, skeletal muscle, and placenta. The mouse protein has been shown to be expressed in capillary endothelial cells. Human CLM-9 mediates the uptake of human IgA2 and mouse IgM.