|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10148-ACG|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10148-ACR|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10148-CF|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10148-CH|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10148-CM|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10148-CY|
|人 CD31/PECAM-1 基因ORF全长cDNA(克隆载体)||HG10148-M|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体)||HG10148-M-N|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10148-NF|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10148-NH|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10148-NM|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10148-NY|
|人 CD31/PECAM-1 基因ORF全长cDNA克隆(表达载体)||HG10148-UT|
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.