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人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签

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Human CDK1 cDNA Clone产品信息
NCBI注册码:NM_001786.4
参考序列ORF长度:894bp
cDNA基因描述:Full length Clone DNA of Homo sapiens cell division cycle 2, G1 to S and G2 to M transcript variant 1 with N terminal His tag.
分子别称:CDK1, CDC28A, MGC111195, DKFZp686L20222, CDC2
分子种属:Human
载体:pCMV3-N-His
质粒:
限制性酶切位点:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签 on other vectors
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10739-ACG1950
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10739-ACR1950
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10739-ANG1950
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10739-ANR1950
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10739-CF1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10739-CH1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10739-CM1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10739-CY1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA(克隆载体)HG10739-M760
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10739-M-F1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG10739-M-N1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10739-NF1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10739-NH1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10739-NM1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10739-NY1770
人 CDC2 Kinase / CDK1 transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG10739-UT1770
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研究背景

CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.

参考资料
  • Lee MG, et al. (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature. 327(6117):31-5.
  • Enserink JM, et al. (2010) An overview of Cdk1-controlled targets and processes. Cell Division. 5(11): 1-41.
  • Ninomiya-Tsuji J, et al. (1991) Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation. Proc Natl Acad Sci. 88(20):9006-10.
  • Zhan Q, et al. (1999) Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45. Oncogene. 18(18):2892-900.
  • Jin S, et al. (2000) The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression. J Biol Chem. 275(22):16602-8.
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