CDC27 cDNA ORF Clone, Human, N-His tag

Cat: HG15970-NH
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CDC27 cDNA ORF Clone, Human, N-His tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
2493 bp
描述
Full length Clone DNA of Human cell division cycle 27 with N terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-N-His
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CDC27 cDNA ORF Clone, Human, N-His tag Alternative Names
ANAPC3 cDNA ORF Clone, Human;APC3 cDNA ORF Clone, Human;CDC27Hs cDNA ORF Clone, Human;D0S1430E cDNA ORF Clone, Human;D17S978E cDNA ORF Clone, Human;H-NUC cDNA ORF Clone, Human;HNUC cDNA ORF Clone, Human;NUC2 cDNA ORF Clone, Human
CDC27 Background Information

Cdc27, a component of the anaphase-promoting complex (APC/C), as a novel binding partner of MCPH1. APC/C is an E3 ubiquitin ligase that controls the cell cycle. A combination of in vitro and in vivo experiments revealed that the interaction between MCPH1 and Cdc27 is dependent on the phosphorylation of Cdc27 and is mediated by the C-terminal BRCT domains of MCPH1.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
cell division cycle 27
References
  • Singh N, Wiltshire TD, Thompson JR, Mer G, Couch FJ. Molecular Basis for the Association of Microcephalin (MCPH1) Protein with the Cell Division Cycle Protein 27 (Cdc27) Subunit of the Anaphase-promoting Complex. The Journal of Biological Chemistry. 2012;287(4):2854-2862.
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