|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10150-ACG|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10150-ACR|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10150-CF|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10150-CH|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10150-CM|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10150-CY|
|人 Cadherin-6/CDH6 基因ORF全长cDNA(克隆载体)||HG10150-M|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10150-M-F|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体)||HG10150-M-N|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10150-NF|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10150-NH|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10150-NM|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10150-NY|
|人 Cadherin-6/CDH6 基因ORF全长cDNA克隆(表达载体)||HG10150-UT|
Cadherins are a family of calcium-dependent, cell-cell adhesion molecules that play an important morphoregulatory role in a wide variety of tissues. Alterations in cadherin function have been implicated in tumor progression in a number of adenocarcinomas. Cadherin-6 (CDH6), also known as K-cadherin (KCAD), is a type-II classic cadherin cell-cell adhesion molecules, which are expressed in graded or areal patterns, as well as layer-specific patterns, in the cortical plate. Human Cadherin-6 is synthesized as a 790 aa type I transmembrane glycoprotein that contains a 18 aa signal peptide, a 35 aa propeptide, a 562 aa extracellular region, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain. There are five cadherin domains of approximately 110 aa each in the extracellular region. Cadherin-6 is highly expressed in brain, cerebellum, and kidney, and may contribute to the formation of the segmental structure of the early brain, as well as the development of renal proximal tubules. Weak expression is also detected lung, pancreas, and gastric mucosa. Additionally, it is specifically expressed in the proximal tubule of normal kidneys and in renal cell cancer. Thus , Cadherin-6 is a new prognostic factor for renal cancer.