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人 CGB7 基因ORF全长cDNA克隆(表达载体), N-Myc 标签

    产品数据评论相关产品实验方法
     CGB7 cDNA Clone产品信息
    NCBI注册码:NM_033043.1
    参考序列ORF长度:498bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens chorionic gonadotropin, beta polypeptide 7 with N terminal Myc tag.
    分子别称:FLJ35403, FLJ43118, CG-beta-a, CGB7
    分子种属:Human
    载体:pCMV3-SP-N-Myc
    质粒:
    限制性酶切位点:
    Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CGB7 qPCR primers for gene expression analysis, HP101741 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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    研究背景

    CGB7 (chorionic gonadotropin, beta polypeptide 7) belongs to the glycoprotein hormones subunit beta family. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CGB7 gene is a member of the glycoprotein hormone beta chain family and encodes the beta 7 subunit of chorionic gonadotropin (CG). CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. CGB7 is used as adjunctive therapy in the treatment of obesity. CGB7 also stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.

    参考资料
  • Fiddes J.C., et al.,(1980), The cDNA for the beta-subunit of human chorionic gonadotropin suggests evolution of a gene by readthrough into the 3'-untranslated region. Nature 286:684-687.
  • Talmadge K., et al., (1984), Evolution of the genes for the beta subunits of human chorionic gonadotropin and luteinizing hormone.Nature 307:37-40.
  • Policastro P., et al.,(1983), The beta subunit of human chorionic gonadotropin is encoded by multiple genes.J. Biol. Chem. 258:11492-11499.
  • Datasheet & Documentation

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