|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG11227-ACG|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG11227-ACR|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG11227-CF|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11227-CH|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG11227-CM|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG11227-CY|
|人 CHI3L1/YKL40 基因ORF全长cDNA(克隆载体)||HG11227-M|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11227-M-H|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG11227-NF|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG11227-NH|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG11227-NM|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG11227-NY|
|人 CHI3L1/YKL40 基因ORF全长cDNA克隆(表达载体)||HG11227-UT|
Chitinase-3-like protein 1 (CHI3L1) is a secreted heparin-binding glycoprotein whose expression is associated with vascular smooth muscle cell migration. CHI3L1 is expressed at high levels in postconfluent nodular VSMC cultures and at low levels in subconfluent proliferating cultures. CHI3L1 is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyto / macrophage markers, its expression is absent in monocytes and strong induced during late stages of human macrophage differentiation. Elevated levels of CHI3L1 are associated with disorders exhibiting increased connective tissue turnover, such as rheum atoid, arthritis, osteoarthritis, scleroderma, and cirrhosis of liver, but is produced in cartilage from old donors or patiens with osteoarthritis. CHI3L1 is abnormally expressed in the hippocampus of subjects with schizophrenia and may be involved in the cellular response to various environmental events that are reported to increase the risk of schizophrenia.