( We provide with CKMT1A qPCR primers for gene expression analysis, HP102587 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13924-ACG|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13924-ACR|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG13924-ANG|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG13924-ANR|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13924-CF|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13924-CH|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13924-CM|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13924-CY|
|人 CKMT1A 基因ORF全长cDNA(克隆载体)||HG13924-G|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13924-NF|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13924-NH|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13924-NM|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13924-NY|
|人 CKMT1A 基因ORF全长cDNA克隆(表达载体)||HG13924-UT|
CKMT1A belongs to the ATP:guanido phosphotransferase family. It contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. CKMT1A gene is one of two genes which encode the ubiquitous mitochondrial creatine kinase (CKMT1). CKMT1 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. CKMT1 occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric CKMT1.