|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10976-ACG|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10976-ACR|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10976-CF|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10976-CH|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10976-CM|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10976-CY|
|人 CLEC1B/CLEC2 基因ORF全长cDNA(克隆载体)||HG10976-M|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10976-NF|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10976-NH|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10976-NM|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10976-NY|
|人 CLEC1B/CLEC2 基因ORF全长cDNA克隆(表达载体)||HG10976-UT|
CLEC1B, also known as CLEC2, is a C-type lectin-like receptor expressed in myeloid cells and NK cells. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC2 contains 1 C-type lectin domain and is expressed preferentially in the liver. It acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.