( We provide with CNDP2 qPCR primers for gene expression analysis, HP100236 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10168-ACG|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10168-ACR|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG10168-ANG|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG10168-ANR|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10168-CF|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10168-CH|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10168-CM|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10168-CY|
|人 CNDP2 / CPGL 基因全长ORF克隆||HG10168-M|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10168-NF|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10168-NH|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10168-NM|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10168-NY|
|人 CNDP2 基因ORF全长cDNA(克隆载体)||HG10168-U|
|人 CNDP2 基因ORF全长cDNA克隆(表达载体)||HG10168-UT|
Cytosolic non-specific dipeptidase, also known as CNDP dipeptidase 2, Glutamate carboxypeptidase-like protein 1, Peptidase A, CNDP2 and CN2, is a cytoplasm protein which belongs to the peptidase M20A family. CNDP2 / CPGL is a cytosolic enzyme that can hydrolyze carnosine to yield l-histidine and beta-alanine. CNDP2 / CPGL hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. It may be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Isoform 1 of CNDP2 / CPGL is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform 2 of CNDP2 / CPGL is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. CNDP2 / CPGL is highly expressed in the histaminergic neurons in the tuberomammillary nucleus, implying that it may supply histidine to histaminergic neurons for histamine synthesis.