|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG14306-ACG|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG14306-ACR|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG14306-ANG|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG14306-ANR|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG14306-CF|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG14306-CH|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG14306-CM|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG14306-CY|
|人 COL4A3BP 基因ORF全长cDNA(克隆载体)||HG14306-G|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG14306-NF|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG14306-NH|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG14306-NM|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG14306-NY|
|人 COL4A3BP 基因ORF全长cDNA克隆(表达载体)||HG14306-UT|
COL4A3BP is a member of the StarD2 subfamily. It contains a pleckstrin homology domain at its amino terminus and a START domain towards the end of the molecule. COL4A3BP has a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. One isoform of COL4A3BP is also involved in ceramide intracellular transport. COL4A3BP specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. An autoimmune response directed at this antigen can cause goodpasture disease.