|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 COMP 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10173-ACG|
|人 COMP 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10173-ACR|
|人 COMP 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10173-CF|
|人 COMP 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10173-CH|
|人 COMP 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10173-CM|
|人 COMP 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10173-CY|
|人 COMP 基因ORF全长cDNA(克隆载体)||HG10173-M|
|人 COMP 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10173-NF|
|人 COMP 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10173-NH|
|人 COMP 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10173-NM|
|人 COMP 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10173-NY|
|人 COMP 基因ORF全长cDNA克隆(表达载体)||HG10173-UT|
Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellular matrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressed primarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with high affinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growth plate organization and function. It is suggested to play a role in cell growth and development, and recent studies have revealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor of apoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.