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人 CPM qPCR引物对

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     CPM qPCR Product Information
    NCBI注册码:
    分子别称:CPM
    产物长度 (bp):
    产品描述:Verified forward and reverse primers for analyzing the quantitative expression of gene
    质控说明:The primer mix has been verified to generate satisfactory qPCR data on Roche LightCycler480
    运输方式:1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions) is shipped at ambiente temperatura.
    储存方法:The lyophilized product is stable for one year from date of receipt when stored at -20℃.
    The suspended product is stable for six months from date of receipt when stored at -20℃.
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    Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.

    Unique Primer Design

    To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.

    Strict Validation Process

    Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.

    Uniform PCR conditions, Saving time and cost

    ~100% amplification curve, ensuring the accuracy of the RNA quantitative

    Carboxypeptidase M/CPM 研究背景

    Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.

     Carboxypeptidase M/CPM 参考资料
  • Deddish PA. et al., 1990, J Biol Chem. 265 (25): 15083-9.
  • Nagae A. et al., 1992, J Neurochem. 59 (6): 2201-12.
  • Skidgel RA. et al., 1996, Immunopharmacology. 32 (1-3): 48-52.
  • Deiteren K. et al., 2009, Clin Chim Acta. 399 (1-2): 24-39.
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    货号: HP101129
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