CRIPT cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG14560-NF
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CRIPT cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
306 bp
描述
Full length Clone DNA of Human cysteine-rich PDZ-binding protein with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-N-FLAG
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CRIPT cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
HSPC139 cDNA ORF Clone, Human;SSMDF cDNA ORF Clone, Human
CRIPT Background Information

CRIPT, also known as cysteine-rich PDZ-binding protein, belongs to the CRIPT family. It interacts with TUBB1. CRIPT also interacts strongly with the PDZ3 domain of members of the DLG4 family. It is involved in the cytoskeletal anchoring of DLG4 in excitatory synapses. CRIPT is highly conserved from mammals to plants and binds selectively to the third PDZ domain (PDZ3) of PSD-95 via its C terminus. n heterologous cells, CRIPT causes a redistribution of PSD-95 to microtubules. In brain, CRIPT colocalizes with PSD-95 in the postsynaptic density and can be coimmunoprecipitated with PSD-95 and tubulin. These findings suggest that CRIPT may regulate PSD-95 interaction with a tubulin-based cytoskeleton in excitatory synapses.

Full Name
cysteine-rich PDZ-binding protein
References
  • Niethammer M. et al., 1998,Neuron. 20 (4): 693-707.
  • Passafaro M. et al., 2000, Nat Neurosci. 2 (12): 1063-9.
  • Piserchio A. et al., 2002, J Biol Chem. 277 (9): 6967-73.
  • Fukunaga Y. et al., 2005, J Biochem. 138 (2): 177-82.
  • CRIPT cDNA ORF Clone, Mouse, Related Products

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    CRIPT cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CRIPT cDNA ORF Clone in Cloning Vector, Human HG14560-G
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    CRIPT cDNA ORF Clone, Human, C-GFPSpark® tag HG14560-ACG
    CRIPT cDNA ORF Clone, Human, N-GFPSpark® tag HG14560-ANG
    CRIPT cDNA ORF Clone, Human, C-OFPSpark® tag HG14560-ACR
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    CRIPT cDNA ORF Clone, Human, C-DDK (Flag®) tag HG14560-CF
    CRIPT cDNA ORF Clone, Human, C-His tag HG14560-CH
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    CRIPT cDNA ORF Clone, Human, C-HA tag HG14560-CY
    CRIPT cDNA ORF Clone, Human, N-HA tag HG14560-NY
    CRIPT cDNA ORF Clone, Human, C-Myc tag HG14560-CM
    CRIPT cDNA ORF Clone, Human, N-Myc tag HG14560-NM
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