( We provide with CRP qPCR primers for gene expression analysis, HP101134 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG11250-ACG|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG11250-ACR|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG11250-CF|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11250-CH|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG11250-CM|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG11250-CY|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA(克隆载体)||HG11250-M|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体)||HG11250-M-N|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG11250-NF|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG11250-NH|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG11250-NM|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG11250-NY|
|人 C-Reactive 蛋白/CRP 基因ORF全长cDNA克隆(表达载体)||HG11250-UT|
C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.