CSK cDNA ORF Clone, Human, N-His tag

Cat: HG10740-NH
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All CSK Reagents
CRO Services
Product recommended

All CSK Reagents
CSK cDNA ORF Clone, Human, N-His tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
1353 bp
描述
Full length Clone DNA of Human c-src tyrosine kinase with N terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-N-His
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CSK Background Information

The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development.

References

Full Name
c-src tyrosine kinase
References
  • Brauninger A. et al.,1992, Gene. 110: 205-11.
  • Sondhi D. et al., 1999, Biochemistry. 38 (34): 11147-55.
  • Ogawa A. et al., 2002, J Biol Chem. 277 (17): 14351-4.
  • Cole PA. et al., 2003, Curr Opin Chem Biol. 7 (5): 580-5.
  • Baumeister U. et al., 2005,EMBO J. 24 (9): 1686-95.
  • CSK cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    CSK cDNA ORF Clone in Cloning Vector, Rat RG82526-U
    CSK Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG82526-ACGLN

    CSK cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CSK cDNA ORF Clone in Cloning Vector, Mouse MG50893-G
    CSK cDNA ORF Clone, Mouse, untagged MG50893-UT
    CSK cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50893-ACG
    CSK Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50893-ACGLN
    CSK cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50893-ANG
    CSK cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50893-ACR
    CSK cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50893-ANR
    CSK cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50893-CF
    CSK cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50893-NF
    CSK cDNA ORF Clone, Mouse, C-His tag MG50893-CH
    CSK cDNA ORF Clone, Mouse, N-His tag MG50893-NH
    CSK cDNA ORF Clone, Mouse, C-HA tag MG50893-CY
    CSK cDNA ORF Clone, Mouse, N-HA tag MG50893-NY
    CSK cDNA ORF Clone, Mouse, C-Myc tag MG50893-CM
    CSK cDNA ORF Clone, Mouse, N-Myc tag MG50893-NM
    CSK qPCR Primer Pairs, Mouse MP200864

    CSK cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CSK cDNA ORF Clone in Cloning Vector, Human HG10740-M
    CSK cDNA ORF Clone, Human, untagged HG10740-UT
    CSK Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10740-ACGLN
    CSK cDNA ORF Clone, Human, C-GFPSpark® tag HG10740-ACG
    CSK cDNA ORF Clone, Human, N-GFPSpark® tag HG10740-ANG
    CSK cDNA ORF Clone, Human, C-OFPSpark® tag HG10740-ACR
    CSK cDNA ORF Clone, Human, N-OFPSpark® tag HG10740-ANR
    CSK cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10740-CF
    CSK cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10740-NF
    CSK cDNA ORF Clone, Human, C-His tag HG10740-CH
    CSK cDNA ORF Clone, Human, C-HA tag HG10740-CY
    CSK cDNA ORF Clone, Human, N-HA tag HG10740-NY
    CSK cDNA ORF Clone, Human, C-Myc tag HG10740-CM
    CSK cDNA ORF Clone, Human, N-Myc tag HG10740-NM
    CSK qPCR Primer Pairs, Human HP100894
    info info
    添加购物车成功! 添加购物车失败!