Chymotrypsin C cDNA ORF Clone, Human, C-His tag

Cat: HG11456-CH

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Chymotrypsin C cDNA ORF Clone, Human, C-His tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
852 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 144G/A not causing the amino acid variation.
描述
Full length Clone DNA of Human chymotrypsin C (caldecrin) with C terminal His tag.

质粒

启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+0.85kb)
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

Chymotrypsin C cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Chymotrypsin C cDNA ORF Clone, Human, C-His tag Validated Images

other Green fluorescent protein / GFP Gene Plasmid Map 5610

Chymotrypsin C cDNA ORF Clone, Human, C-His tag Alternative Names

CLCR cDNA ORF Clone, Human;ELA4 cDNA ORF Clone, Human

Chymotrypsin C Background Information

Chymotrypsin C (abbreviated for CTRC), also known as caldecrin or elastase4, is a digestive enzyme of the peptidase S1 family. This enzyme is synthesized as an inactivate chymotrypsinogen. On cleavage by trypsin into two parts that activate each other by removing two small peptides in a trans-proteolysis, chymotrypsin C produced. N-linked glycosylation of human CTRC is required for efficient folding and secretion, however, the N-linked glycan is unimportant for enzyme activity or inhibitor binding. It has been proposed that CTRC is a key regulator of digestive zymogen activation and a physiological co-activator of digestive carboxypeptidases proCPA1 and proCPA2. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis. It's speculated that CTRC might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression. The pancreatic cancer cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed CTRC, whereas the cell migration ability was upregulated in Aspc-1 cells in which CTRC was suppressed. 

Full Name
chymotrypsin C (caldecrin)
References
  • Lacruz RS, et al. (2011) Chymotrypsin C (caldecrin) is associated with enamel development. J Dent Res. 90 (10): 1228-33.
  • Zhou J, et al. (2011) Chymotrypsin C mutations in chronic pancreatitis. J Gastroenterol Hepatol. 26 (8): 1238-46.
  • Wang H, et al. (2011) Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration. Acta Biochim Biophys Sin (Shanghai). 43 (5): 362-71.
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