( We provide with CTSB qPCR primers for gene expression analysis, HP100505 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10483-ACG|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10483-ACR|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10483-CF|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10483-CH|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10483-CM|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10483-CY|
|人 CTSB 基因全长ORF克隆||HG10483-M|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10483-NF|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10483-NH|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10483-NM|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10483-NY|
|人 Cathepsin B/CTSB 基因ORF全长cDNA(克隆载体)||HG10483-U|
|人 Cathepsin B/CTSB 基因ORF全长cDNA克隆(表达载体)||HG10483-UT|
Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.