( We provide with CTSC qPCR primers for gene expression analysis, HP100506 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10484-ACG|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10484-ACR|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10484-CF|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10484-CH|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10484-CM|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10484-CY|
|人 Cathepsin C/CTSC 基因ORF全长cDNA(克隆载体)||HG10484-G|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10484-NF|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10484-NH|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10484-NM|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10484-NY|
|人 Cathepsin C/CTSC 基因ORF全长cDNA克隆(表达载体)||HG10484-UT|
Cathepsins are proteases found in many types of cells conserved in all animals, which have a vital role in mammalian cellular turnover such as bone resorption. The lysosomal cysteine protease Cathepsin C (CTSC), also known as dipeptidyl peptidase I (DPPI/DPP1), activates a number of granule-associated serine proteases with pro-inflammatory and immune functions by removal of their inhibitory N-terminal dipeptides. This lysosomal exo-cysteine protease belonging to the peptidase C1 family. Active cathepsin C is found in lysosomes as a 200-kDa multimeric enzyme. Subunits constituting this assembly all arise from the proteolytic cleavage of a single precursor giving rise to three peptides: the propeptide, the alpha- and the beta-chains. It is a central coordinator for activation of many serine proteases in immune/inflammatory cells. Defects in the Cathepsin C have been shown to be a cause of Papillon-Lefevre disease, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis. Cathepsin C plays a key role in the activation of several degradative enzymes linked to tissue destruction in inflammatory diseases. Thus, it is a therapeutic target for the treatment of a number of inflammatory and autoimmune diseases.