( We provide with CXADR qPCR primers for gene expression analysis, HP100713 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10799-ACG|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10799-ACR|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10799-CF|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10799-CH|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10799-CM|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10799-CY|
|人 CXADR / CAR 基因ORF全长cDNA(克隆载体)||HG10799-M|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10799-NF|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10799-NH|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10799-NM|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10799-NY|
|人 CXADR / CAR 基因ORF全长cDNA克隆(表达载体)||HG10799-UT|
CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.