|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG11223-ACG|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG11223-ACR|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG11223-CF|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11223-CH|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG11223-CM|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG11223-CY|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA(克隆载体)||HG11223-M|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG11223-NF|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG11223-NH|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG11223-NM|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG11223-NY|
|人 Chitotriosidase/Chitinase 1/CHIT1 基因ORF全长cDNA克隆(表达载体)||HG11223-UT|
Chitotriosidase, also known as Chitinase-1 and CHIT1, is a member of the glycosyl hydrolase 18 family and Chitinase class II subfamily. It is a member of the mammalian chitinase family, structurally homologous to chitinases from other species, is synthesized and secreted by specifically activated macrophages. Chitotriosidase is a polymer of N-acetylglucosamine. Serum and plasma chitotriosidase activity is usually measured as the first step in diagnosis of Gaucher disease. Monitoring chitotriosidase activity is widely used during treatment of this pathology by enzyme replacement therapy. Its elevated plasma level reflects gradual intralysosomal accumulation in Gaucher cells (lipid-loaded macrophages). Macrophages overloaded by the enzyme accumulated in lysosomal material (lipids) were shown to secrete chitotriosidase; its increased expression was noted in several lysosomal storage diseases and atherosclerosis. In addition to lipid storage disorders, where Chit activity has longer been used as a marker of disease activity and therapeutic response, elevation of plasma Chit may occur in hematological disorders with storage of erythrocyte membrane breakdown products as thalassemia and different systemic infectious diseases sustained by fungi and other pathogens. Recently, increased Chit activity was demonstrated in CNS from patients with different neurological disorders. Chitotriosidase is believed to play a role in mechanisms of immunity and protection against chitin-containing pathogens.