Cystatin A cDNA ORF Clone, Human, C-HA tag

Cat: HG10479-CY
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Cystatin A cDNA ORF Clone, Human, C-HA tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
297 bp
描述
Full length Clone DNA of Human cystatin A (stefin A) with C terminal HA tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-HA
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Cystatin A cDNA ORF Clone, Human, C-HA tag Alternative Names
AREI cDNA ORF Clone, Human;STF1 cDNA ORF Clone, Human;STFA cDNA ORF Clone, Human
Cystatin A Background Information

Cystatin-A, also known as Cystatin-AS, Stefin-A and CSTA, is a cytoplasm protein which belongs to the cystatin family. Cystatin-A / CSTA is a cysteine proteinase inhibitor with a molecular mass of 11 kDa, and is located mainly in the keratohyaline granules of the stratum granulosum and the cornified envelope of the stratum corneum in the epidermis. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).

Full Name
cystatin A
References
  • Machleidt W., et al.,(1983), Protein inhibitors of cysteine proteinases. II. Primary structure of stefin, a cytosolic protein inhibitor of cysteine proteinases from human polymorphonuclear granulocytes. Hoppe-Seyler's Z. Physiol. Chem. 364:1481-1486.
  • Takeda A., et al., (1989), Comparative studies on the primary structure of human cystatin as from epidermis, liver, spleen, and leukocytes.J. Biochem. 105:986-991.
  • Kartasova T., et al.,(1987), Effects of UV, 4-NQO and TPA on gene expression in cultured human epidermal keratinocytes.Nucleic Acids Res. 15:5945-5962.
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