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人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), C-HA 标签

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     DBH cDNA Clone产品信息
    NCBI注册码:BC017174
    参考序列ORF长度:1812bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens dopamine beta-hydroxylase (dopamine beta-monooxyge) with C terminal HA tag.
    分子别称:DBM, DBH
    分子种属:Human
    载体:pCMV3-C-HA
    质粒:
    限制性酶切位点:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with DBH qPCR primers for gene expression analysis, HP102142 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), C-HA 标签 on other vectors
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG13440-ACG2070
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG13440-ACR2070
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG13440-ANG2070
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG13440-ANR2070
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG13440-CF1890
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), C-His 标签HG13440-CH1890
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG13440-CM1890
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG13440-CY1890
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA(克隆载体)HG13440-G920
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG13440-NF1890
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), N-His 标签HG13440-NH1890
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG13440-NM1890
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG13440-NY1890
    人 DBH / Dopamine beta-Hydroxylase 基因ORF全长cDNA克隆(表达载体)HG13440-UT1890
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    研究背景

    DBH is a 290 kDa copper-containing oxygenase. It can be detected in noradrenergic nerve terminals of the central and peripheral nervous systems, and is also expressed in chromaffin cells of the adrenal medulla. DBH contains our identical subunits, and its activity requires ascorbate as a cofactor. It functions in in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine the only transmitter synthesized inside vesicles. DBH has been shown to be associated with decision making and addictive behaviors such as alcohol and smoking, attention deficit hyperactivity disorder, and also with neurological diseases such as Schizophrenia and Alzheimer's.

    参考资料
  • Rush RA. et al., 1980, Crit Rev Clin Lab Sci. 12 (3): 241-77.
  • Goldstein M. et al., 1964, Life Sci. 3 (7): 763-7.
  • S Friedman. et al., 1966, The Journal of Biological Chemistry. 241 (10): 2256-9.
  • Size / Price
    货号: HG13440-CY
    目录价: 
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