DICER1 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG11350-CF

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DICER1 cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
5769 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 12T>C not causing the amino acid variation.
描述
Full length Clone DNA of Human dicer 1, ribonuclease type I II with C terminal Flag tag.

质粒

启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + NotI (6kb + 5.81kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

DICER1 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

DICER1 cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images

Human DICER1 Gene Expression validated Image 1787
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
Cynomolgus CDCP1 Gene Plasmid Map 5627

DICER1 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names

DCR1 cDNA ORF Clone, Human;Dicer cDNA ORF Clone, Human;Dicer1e cDNA ORF Clone, Human;HERNA cDNA ORF Clone, Human;K12H4.8-LIKE cDNA ORF Clone, Human;MNG1 cDNA ORF Clone, Human;RMSE2 cDNA ORF Clone, Human

DICER1 Background Information

Full Name
dicer 1, ribonuclease type III
info info
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