( We provide with DSC2 qPCR primers for gene expression analysis, HP100913 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 DSC2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10809-ACG|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10809-ACR|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10809-CF|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10809-CH|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10809-CM|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10809-CY|
|人 DSC2 基因ORF全长cDNA(克隆载体)||HG10809-M|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10809-NF|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10809-NH|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10809-NM|
|人 DSC2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10809-NY|
|人 DSC2 基因ORF全长cDNA克隆(表达载体)||HG10809-UT|
DSC2 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. Like other desmocollins, murine DSC2 has two products, Dsc2a and Dsc2b, produced by alternative splicing of a 46 bp exon which encodes 11 COOH-terminal aa followed by an in-frame stop codon. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in DSC2 are associated with arrhythmogenic right ventricular dysplasia-11. DSC2 is Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSC2 may contribute to epidermal cell positioning by mediating differential adhesiveness between cells that express different isoforms.