E-Cadherin cDNA ORF Clone, Human, C-HA tag

Cat: HG10204-CY

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E-Cadherin cDNA ORF Clone, Human, C-HA tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
2649 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutation 2076 T/C not causing the amino acid variation.
描述
Full length Clone DNA of Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) with C terminal HA tag.

质粒

启动子
Enhanced CMV promoter
载体
限制性酶切位点
HindIII + XbaI (6kb + 2.7kb)
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

E-Cadherin cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

E-Cadherin cDNA ORF Clone, Human, C-HA tag Validated Images

Cynomolgus CD19/B4/CVID3 Gene Plasmid Map 5622

E-Cadherin cDNA ORF Clone, Human, C-HA tag Alternative Names

Arc-1 cDNA ORF Clone, Human;CD324 cDNA ORF Clone, Human;CDH1 cDNA ORF Clone, Human;CDHE cDNA ORF Clone, Human;E-cad cDNA ORF Clone, Human;E-Cadherin cDNA ORF Clone, Human;ECAD cDNA ORF Clone, Human;LCAM cDNA ORF Clone, Human;UVO cDNA ORF Clone, Human

E-Cadherin Background Information

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Full Name
cadherin 1, type 1, E-cadherin (epithelial)
References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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