快速订购

人 EDNRB / Endothelin B Receptor 基因ORF全长cDNA克隆(表达载体), N-Myc 标签

  • Ferret CD3e/CD3 epsilon Gene Plasmid Map 5630
产品数据评论相关产品实验方法
 EDNRB cDNA Clone产品信息
NCBI注册码:BC014472
参考序列ORF长度:1344 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens endothelin receptor type B with N terminal Myc tag.
分子别称:ETB, ET-B, ETBR, ETRB, HSCR, WS4A, ABCDS, ET-BR, HSCR2
分子种属:Human
载体:pCMV3-SP-N-Myc
质粒:pCMV3-SP-Myc-EDNRB
限制性酶切位点:KpnI + XbaI(6kb+1.34kb)
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with EDNRB qPCR primers for gene expression analysis, HP104096 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Product nameProduct name
Size / Price
货号: HG15473-NM
目录价: 
单价:      (You Save: )
规格:
数量:+-
库存In Stock
加入购物车大包装查询

Datasheet & Documentation

Contact Us
    您曾浏览过的产品
      产品数据可能随批次变化,最新数据请详见随货说明书。
      请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”