( We provide with EphB3 qPCR primers for gene expression analysis, HP102590 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13925-ACG|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13925-ACR|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13925-CF|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13925-CH|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13925-CM|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13925-CY|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA(克隆载体)||HG13925-G|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13925-NF|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13925-NH|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13925-NM|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13925-NY|
|人 EphB3/HEK2/Eph Receptor B3 基因ORF全长cDNA克隆(表达载体)||HG13925-UT|
Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.