EphB3 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG13925-CF
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All EphB3 Reagents
CRO Services
Product recommended

All EphB3 Reagents
EphB3 cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
3036 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1053T/C not causing the amino acid variation.
描述
Full length Clone DNA of Human EPH receptor B3 with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
限制性酶切位点
KpnI + XbaI(6kb+3.04kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
EphB3 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

EphB3 cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
other Green fluorescent protein / GFP Gene Plasmid Map 5609
EphB3 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
ETK2 cDNA ORF Clone, Human;HEK2 cDNA ORF Clone, Human;TYRO6 cDNA ORF Clone, Human
EphB3 Background Information

Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Full Name
EPH receptor B3
References
  • Bergemann AD, et al. (1998) Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene. 16(4): 471-80.
  • Hock B, et al. (1998) PDZ-domain-mediated interaction of the Eph-related receptor tyrosine kinase EphB3 and the ras-binding protein AF6 depends on the kinase activity of the receptor. Proc Natl Acad Sci U S A. 95(17): 9779-84.
  • Liu X, et al. (2006) EphB3: an endogenous mediator of adult axonal plasticity and regrowth after CNS injury. J Neurosci. 26(12): 3087-101.
  • EphB3 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    EphB3 cDNA ORF Clone in Cloning Vector, Mouse MG50581-M
    EphB3 cDNA ORF Clone, Mouse, untagged MG50581-UT
    EphB3 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50581-ACG
    EphB3 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50581-ACGLN
    EphB3 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50581-ACR
    EphB3 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50581-CF
    EphB3 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50581-NF
    EphB3 cDNA ORF Clone, Mouse, C-His tag MG50581-CH
    EphB3 cDNA ORF Clone, Mouse, N-His tag MG50581-NH
    EphB3 cDNA ORF Clone, Mouse, C-HA tag MG50581-CY
    EphB3 cDNA ORF Clone, Mouse, N-HA tag MG50581-NY
    EphB3 cDNA ORF Clone, Mouse, C-Myc tag MG50581-CM
    EphB3 cDNA ORF Clone, Mouse, N-Myc tag MG50581-NM
    EphB3 qPCR Primer Pairs, Mouse MP200573

    EphB3 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    EphB3 cDNA ORF Clone in Cloning Vector, Human HG13925-G
    EphB3 cDNA ORF Clone, Human, untagged HG13925-UT
    EphB3 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13925-ACGLN
    EphB3 cDNA ORF Clone, Human, C-GFPSpark® tag HG13925-ACG
    EphB3 cDNA ORF Clone, Human, C-OFPSpark® tag HG13925-ACR
    EphB3 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG13925-NF
    EphB3 cDNA ORF Clone, Human, C-His tag HG13925-CH
    EphB3 cDNA ORF Clone, Human, N-His tag HG13925-NH
    EphB3 cDNA ORF Clone, Human, C-HA tag HG13925-CY
    EphB3 cDNA ORF Clone, Human, N-HA tag HG13925-NY
    EphB3 cDNA ORF Clone, Human, C-Myc tag HG13925-CM
    EphB3 cDNA ORF Clone, Human, N-Myc tag HG13925-NM
    EphB3 qPCR Primer Pairs, Human HP102590
    info info
    添加购物车成功! 添加购物车失败!