EpCAM cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10694-CF

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All EpCAM Reagents

CRO Services

Product recommended


All EpCAM Reagents

EpCAM cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
945 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human tumor-associated calcium signal transducer 1 with C terminal Flag tag.

质粒

启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI (6kb + 1kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

EpCAM cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

EpCAM cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images

Human EpCAM/TROP-1/TACSTD1 Gene Expression validated Image 1596
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
other Green fluorescent protein / GFP Gene Plasmid Map 5609

EpCAM cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names

DIAR5 cDNA ORF Clone, Human;EGP-2 cDNA ORF Clone, Human;EGP314 cDNA ORF Clone, Human;EGP40 cDNA ORF Clone, Human;ESA cDNA ORF Clone, Human;HNPCC8 cDNA ORF Clone, Human;KS1/4 cDNA ORF Clone, Human;KSA cDNA ORF Clone, Human;M4S1 cDNA ORF Clone, Human;MIC18 cDNA ORF Clone, Human;MK-1 cDNA ORF Clone, Human;TACSTD1 cDNA ORF Clone, Human;TROP-1 cDNA ORF Clone, Human;TROP1 cDNA ORF Clone, Human

EpCAM Background Information

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
epithelial cell adhesion molecule
References
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
  • EpCAM cDNA ORF Clone, Rhesus, Related Products

    Product Name Catalog#(PDF)
    EpCAM cDNA ORF Clone in Cloning Vector, Rhesus CG90299-G
    EpCAM cDNA ORF Clone, Rhesus, untagged CG90299-UT
    EpCAM Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90299-ACGLN
    EpCAM cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90299-ACG
    EpCAM cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90299-ACR
    EpCAM cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90299-CF
    EpCAM cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90299-NF
    EpCAM cDNA ORF Clone, Rhesus, C-His tag CG90299-CH
    EpCAM cDNA ORF Clone, Rhesus, N-His tag CG90299-NH
    EpCAM cDNA ORF Clone, Rhesus, C-HA tag CG90299-CY
    EpCAM cDNA ORF Clone, Rhesus, N-HA tag CG90299-NY
    EpCAM cDNA ORF Clone, Rhesus, C-Myc tag CG90299-CM
    EpCAM cDNA ORF Clone, Rhesus, N-Myc tag CG90299-NM

    EpCAM cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    EpCAM cDNA ORF Clone in Cloning Vector, Rat RG80306-G
    EpCAM cDNA ORF Clone, Rat, untagged RG80306-UT
    EpCAM cDNA ORF Clone, Rat, C-GFPSpark® tag RG80306-ACG
    EpCAM Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80306-ACGLN
    EpCAM cDNA ORF Clone, Rat, C-OFPSpark® tag RG80306-ACR
    EpCAM cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80306-CF
    EpCAM cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80306-NF
    EpCAM cDNA ORF Clone, Rat, C-His tag RG80306-CH
    EpCAM cDNA ORF Clone, Rat, N-His tag RG80306-NH
    EpCAM cDNA ORF Clone, Rat, C-HA tag RG80306-CY
    EpCAM cDNA ORF Clone, Rat, N-HA tag RG80306-NY
    EpCAM cDNA ORF Clone, Rat, C-Myc tag RG80306-CM
    EpCAM cDNA ORF Clone, Rat, N-Myc tag RG80306-NM
    EpCAM qPCR Primer Pairs, Rat RP300282

    EpCAM cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    EpCAM cDNA ORF Clone in Cloning Vector, Human HG10694-G
    EpCAM cDNA ORF Clone, Human, untagged HG10694-UT
    EpCAM cDNA ORF Clone, Human, C-GFPSpark® tag HG10694-ACG
    EpCAM Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10694-ACGLN
    EpCAM cDNA ORF Clone, Human, C-OFPSpark® tag HG10694-ACR
    EpCAM cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10694-NF
    EpCAM cDNA ORF Clone, Human, C-His tag HG10694-CH
    EpCAM cDNA ORF Clone, Human, N-His tag HG10694-NH
    EpCAM cDNA ORF Clone, Human, C-HA tag HG10694-CY
    EpCAM cDNA ORF Clone, Human, N-HA tag HG10694-NY
    EpCAM cDNA ORF Clone, Human, C-Myc tag HG10694-CM
    EpCAM cDNA ORF Clone, Human, N-Myc tag HG10694-NM
    EpCAM qPCR Primer Pairs, Human HP100650
    info info
    添加购物车成功! 添加购物车失败!