Factor XIII cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG11510-NF

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Factor XIII cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
2013 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human coagulation factor XIII, B polypeptide with N terminal Flag tag.

质粒

启动子
Enhanced CMV promoter
限制性酶切位点
KpnI(two restriction sites) + XbaI(6kb+0.81kb+1.21kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

Factor XIII cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Factor XIII cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names

Coagulation factor 13 cDNA ORF Clone, Human;Coagulation factor XIII cDNA ORF Clone, Human;FXIIIB cDNA ORF Clone, Human

Factor XIII Background Information

Coagulation factor XIII B chain, also known as Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain and F13B, is a secreted protein which contains 10 Sushi ( CCP / SCR ) domains. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Factor XIII acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Defects in F13B are the cause of factor XIII subunit B deficiency ( FA13BD ) which is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.

Full Name
coagulation factor XIII, B polypeptide
References
  • Bottenus R.E., et al.,(1990), Nucleotide sequence of the gene for the b subunit of human factor XIII. Biochemistry 29:11195-11209.
  • Ichinose A., et al., (1986), Amino acid sequence of the b subunit of human factor XIII, a protein composed of ten repetitive segments.Biochemistry 25:4633-4638.
  • Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.
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