Factor IX cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG11503-NF

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Factor IX cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
1386 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human coagulation factor IX with N terminal Flag tag.

质粒

启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI (6kb + 1.39kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

Factor IX cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Factor IX cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images

Rhesus CD64/Fc gamma RI Gene Plasmid Map 5614

Factor IX cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names

Coagulation factor 9 cDNA ORF Clone, Human;Coagulation factor IX cDNA ORF Clone, Human;FIX cDNA ORF Clone, Human;HEMB cDNA ORF Clone, Human;P19 cDNA ORF Clone, Human;PTC cDNA ORF Clone, Human;THPH8 cDNA ORF Clone, Human

Factor IX Background Information

Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.

Full Name
coagulation factor IX
References
  • Onay U.V., et al., 2003, Br. J. Haematol. 120:656-659.
  • Vidal F., et al., 2000, Br. J. Haematol. 111:549-551.
  • Simioni P., et al., 2009, N. Engl. J. Med. 361:1671-1675.
  • Espinos C., et al., 2009, Haematologica 88:235-236.
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    Factor IX cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
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    Factor IX cDNA ORF Clone, Human, C-OFPSpark® tag HG11503-ACR
    Factor IX cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11503-CF
    Factor IX cDNA ORF Clone, Human, C-His tag HG11503-CH
    Factor IX cDNA ORF Clone, Human, N-His tag HG11503-NH
    Factor IX cDNA ORF Clone, Human, C-HA tag HG11503-CY
    Factor IX cDNA ORF Clone, Human, N-HA tag HG11503-NY
    Factor IX cDNA ORF Clone, Human, C-Myc tag HG11503-CM
    Factor IX cDNA ORF Clone, Human, N-Myc tag HG11503-NM
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