Human FAM19A4 HEK293 Overexpression Lysate

Cat: 11165-H01HL
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Human FAM19A4 HEK293 Overexpression Lysate 产品信息
Product Description
This Human FAM19A4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of FAM19A4 protein (Cat: 11165-H01H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human FAM19A4 (Q96LR4) (Ser36-Arg140) was expressed with the Fc region of human IgG1 at the N-terminus.
Molecule Mass
The recombinant human FAM19A4 /Fc comprises 365 amino acids and has a predicted molecular mass of 40.1 kDa. The apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions.
Human FAM19A4 HEK293 Overexpression Lysate Usage Guide
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Human FAM19A4 HEK293 Overexpression Lysate Alternative Names
Human TAFA-4 Overexpression Lysate;Human TAFA4 Overexpression Lysate
FAM19A4 Background Information
Full Name
family with sequence similarity 19 (chemokine (C-C motif)-like), member A4
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