Fc epsilon RI cDNA ORF Clone, Human, N-HA tag

Cat: HG13193-NY

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Fc epsilon RI cDNA ORF Clone, Human, N-HA tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
789 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide with N terminal HA tag.

质粒

启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+0.79kb)
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

Fc epsilon RI cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Fc epsilon RI cDNA ORF Clone, Human, N-HA tag Validated Images

Rhesus CD3d/CD3 delta Gene Plasmid Map 5616

Fc epsilon RI cDNA ORF Clone, Human, N-HA tag Alternative Names

FCE1A cDNA ORF Clone, Human;FcERI cDNA ORF Clone, Human

Fc epsilon RI Background Information

FcERI, also known as FCER1A, is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit(FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.

Full Name
Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
References
  • Shikanai T, et al. (2002) Sequence variants in the FcepsilonRI alpha chain gene. J Appl Physiol. 93(1):37-41.
  • Sada K, et al. (2002) Regulation of FcepsilonRI-mediated degranulation by an adaptor protein 3BP2 in rat basophilic leukemia RBL-2H3 cells. Blood. 100(6):2138-44.
  • Takahashi K, et al. (2003) Transcriptional regulation of the human high affinity IgE receptor alpha-chain gene. Mol Immunol. 38(16-18):1193-9.
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    Fc epsilon RI cDNA ORF Clone, Human, Related Products

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