FGF10 cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG10573-NF
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All FGF10 Reagents
CRO Services
Product recommended

All FGF10 Reagents
FGF10 cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
603 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human fibroblast growth factor 10 with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-SP-N-FLAG
限制性酶切位点
KpnI + XbaI (6kb + 0.6kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
FGF10 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

FGF10 cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
Rhesus CD64/Fc gamma RI Gene Plasmid Map 5614
Human FGF10 / KGF2 Gene Expression validated Image 16186
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
FGF10 cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
KGF2 cDNA ORF Clone, Human
FGF10 Background Information

Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.

Full Name
fibroblast growth factor 10
References
  • Sekine K, et al. (1999) Fgf10 is essential for limb and lung formation. Nat Genet. 21(1): 138-41.
  • Ohuchi H, et al. (2000) FGF10 acts as a major ligand for FGF receptor 2 IIIb in mouse multi-organ development. Biochem Biophys Res Commun. 277(3): 643-9.
  • Bellusci S, et al. (1997) Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung. Development. 124(23): 4867-78.
  • FGF10 cDNA ORF Clone, Rhesus, Related Products

    Product Name Catalog#(PDF)
    FGF10 cDNA ORF Clone in Cloning Vector, Rhesus CG90087-G
    FGF10 cDNA ORF Clone, Rhesus, untagged CG90087-UT
    FGF10 cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90087-ACG
    FGF10 Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90087-ACGLN
    FGF10 cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90087-ACR
    FGF10 cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90087-CF
    FGF10 cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90087-NF
    FGF10 cDNA ORF Clone, Rhesus, C-His tag CG90087-CH
    FGF10 cDNA ORF Clone, Rhesus, N-His tag CG90087-NH
    FGF10 cDNA ORF Clone, Rhesus, C-HA tag CG90087-CY
    FGF10 cDNA ORF Clone, Rhesus, N-HA tag CG90087-NY
    FGF10 cDNA ORF Clone, Rhesus, C-Myc tag CG90087-CM
    FGF10 cDNA ORF Clone, Rhesus, N-Myc tag CG90087-NM

    FGF10 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    FGF10 cDNA ORF Clone, Rat, untagged RG82712-UT
    FGF10 cDNA ORF Clone, Rat, C-GFPSpark® tag RG82712-ACG
    FGF10 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG82712-ACGLN
    FGF10 cDNA ORF Clone, Rat, C-OFPSpark® tag RG82712-ACR
    FGF10 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG82712-CF
    FGF10 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG82712-NF
    FGF10 cDNA ORF Clone, Rat, C-His tag RG82712-CH
    FGF10 cDNA ORF Clone, Rat, N-His tag RG82712-NH
    FGF10 cDNA ORF Clone, Rat, C-HA tag RG82712-CY
    FGF10 cDNA ORF Clone, Rat, N-HA tag RG82712-NY
    FGF10 cDNA ORF Clone, Rat, C-Myc tag RG82712-CM
    FGF10 cDNA ORF Clone, Rat, N-Myc tag RG82712-NM

    FGF10 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    FGF10 cDNA ORF Clone in Cloning Vector, Mouse MG50196-M
    FGF10 cDNA ORF Clone, Mouse, untagged MG50196-UT
    FGF10 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50196-ACG
    FGF10 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50196-ACGLN
    FGF10 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50196-ACR
    FGF10 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50196-CF
    FGF10 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50196-NF
    FGF10 cDNA ORF Clone, Mouse, C-His tag MG50196-CH
    FGF10 cDNA ORF Clone, Mouse, N-His tag MG50196-NH
    FGF10 cDNA ORF Clone, Mouse, C-HA tag MG50196-CY
    FGF10 cDNA ORF Clone, Mouse, N-HA tag MG50196-NY
    FGF10 cDNA ORF Clone, Mouse, C-Myc tag MG50196-CM
    FGF10 cDNA ORF Clone, Mouse, N-Myc tag MG50196-NM
    FGF10 qPCR Primer Pairs, Mouse MP200226

    FGF10 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    FGF10 cDNA ORF Clone in Cloning Vector, Human HG10573-M
    FGF10 cDNA ORF Clone, Human, untagged HG10573-UT
    FGF10 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10573-ACGLN
    FGF10 cDNA ORF Clone, Human, C-GFPSpark® tag HG10573-ACG
    FGF10 cDNA ORF Clone, Human, C-OFPSpark® tag HG10573-ACR
    FGF10 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10573-CF
    FGF10 cDNA ORF Clone, Human, C-His tag HG10573-CH
    FGF10 cDNA ORF Clone, Human, N-His tag HG10573-NH
    FGF10 cDNA ORF Clone, Human, C-HA tag HG10573-CY
    FGF10 cDNA ORF Clone, Human, N-HA tag HG10573-NY
    FGF10 cDNA ORF Clone, Human, C-Myc tag HG10573-CM
    FGF10 cDNA ORF Clone, Human, N-Myc tag HG10573-NM
    FGF10 qPCR Primer Pairs, Human HP100580
    info info
    添加购物车成功! 添加购物车失败!