输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!

快速订购

Text Size:AAA

人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), N-Flag 标签

产品数据评论相关产品实验方法
Human FUOM cDNA Clone产品信息
NCBI注册码:NM_001098483.1
参考序列ORF长度:465bp
cDNA基因描述:Full length Clone DNA of Homo sapiens fucose mutarotase with N terminal Flag tag.
分子别称:FUCU, FucM, C10orf125
分子种属:Human
载体:pCMV3-N-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), N-Flag 标签 on other vectors
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG13974-ACG1950
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG13974-ACR1950
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG13974-ANG1950
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG13974-ANR1950
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG13974-CF1770
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), C-His 标签HG13974-CH1770
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG13974-CM1770
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG13974-CY1770
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA(克隆载体)HG13974-G760
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG13974-NF1770
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), N-His 标签HG13974-NH1770
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG13974-NM1770
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG13974-NY1770
人 FUOM / fucose mutarotase / FucM 基因ORF全长cDNA克隆(表达载体)HG13974-UT1770
 点击了解更多载体信息
Product nameProduct name
研究背景

FUOM, also known as fucose mutarotase and FucM, belongs to the RbsD / FucU family. FUOM is involved in the interconversion between alpha- and beta-L-fucoses. L-Fucose has two isforms: alpha-L-fucose (29.5%) and beta-L-fucose (70.5%). The beta-form is metabolized through the salvage pathway. GDP-L-fucose formed either by the de novo or salvage pathways is transported into the endoplasmic reticulum, where it serves as a substrate for N- and O-glycosylations by fucosyltransferases. Fucosylated structures expressed on cell surfaces or secreted in biological fluids are believed to play a critical role in cell-cell adhesion and recognition processes. FUOM mainly exists as homodimer, but also functions as homotetramer, homooctamer, and homodecamer. FUOM's homodimeric form seems catalytically inactive.

参考资料
  • Deloukas P. et al., 2004, Nature. 429: 375-81.
  • Ota T. et al., 2004, Nat Genet. 36: 40-5.
  • Dongkyu Park. et al., 2007, Glycobiology. 17 (9): 955-62.
  • Size / Price
    货号: HG13974-NF
    目录价: 
    单价:      (You Save: )
    规格:
    数量:+-
    库存2-3 weeks
    大包装查询加入购物车
    产品数据可能随批次变化,最新数据请详见随货说明书。
    请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”