Factor VII cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG11506-NF

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All Factor VII Reagents

CRO Services

Product recommended


All Factor VII Reagents

Factor VII cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
1335 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 486 C/T, 1236 C/T not causing the amino acid variation.
描述
Full length Clone DNA of Human coagulation factor VII (serum prothrombin conversion accelerator) with N terminal Flag tag.

质粒

启动子
Enhanced CMV promoter
限制性酶切位点
HindIII + NotI (6kb + 1.36kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

Factor VII cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Factor VII cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images

Human CD112/Nectin-2/PVRL2 Gene Plasmid Map 5634
Human Coagulation Factor VII Gene Expression validated Image 16399
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

Factor VII cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names

Coagulation factor 7 cDNA ORF Clone, Human;Coagulation factor VII cDNA ORF Clone, Human;SPCA cDNA ORF Clone, Human

Factor VII Background Information

Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class, and Factor VII (FVII) deficiency is the most frequent among rare congenital bleeding disorders. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Recombinant activated factor VII (rFVIIa) is a haemostatic agent, which was originally developed for the treatment of haemophilia patients with inhibitors against factor FVIII or FIX. FVIIa binds specifically to endothelial protein C receptor (EPCR), a known cellular receptor for protein C and activated protein C, on the endothelium. rFVIIa is a novel hemostatic agent, originally developed for the treatment of hemorrhage in hemophiliacs with inhibitors, which has been successfully used recently in an increasing number of nonhemophilic bleeding conditions.

Full Name
coagulation factor VII (serum prothrombin conversion accelerator)
References
  • Franchini M, et al. (2007) Potential role of recombinant activated factor VII for the treatment of severe bleeding associated with disseminated intravascular coagulation: a systematic review. Blood Coagul Fibrinolysis. 18(7): 589-93.
  • Lapecorella M, et al. (2008) Factor VII deficiency: defining the clinical picture and optimizing therapeutic options. Haemophilia. 14(6): 1170-5.
  • Grottke O, et al. (2010) Activated recombinant factor VII (rFVIIa). Best Pract Res Clin Anaesthesiol. 24(1): 95-106.
  • Factor VII cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    Factor VII cDNA ORF Clone in Cloning Vector, Mouse MG50034-M
    Factor VII cDNA ORF Clone, Mouse, untagged MG50034-UT
    Factor VII cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50034-ACG
    Factor VII Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50034-ACGLN
    Factor VII cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50034-ACR
    Factor VII cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50034-CF
    Factor VII cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50034-NF
    Factor VII cDNA ORF Clone, Mouse, C-His tag MG50034-CH
    Factor VII cDNA ORF Clone, Mouse, N-His tag MG50034-NH
    Factor VII cDNA ORF Clone, Mouse, C-HA tag MG50034-CY
    Factor VII cDNA ORF Clone, Mouse, N-HA tag MG50034-NY
    Factor VII cDNA ORF Clone, Mouse, C-Myc tag MG50034-CM
    Factor VII cDNA ORF Clone, Mouse, N-Myc tag MG50034-NM
    Factor VII qPCR Primer Pairs, Mouse MP200034

    Factor VII cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    Factor VII cDNA ORF Clone in Cloning Vector, Human HG14137-G
    Factor VII cDNA ORF Clone in Cloning Vector, Human HG11506-M
    Factor VII cDNA ORF Clone, Human, untagged HG14137-UT
    Factor VII cDNA ORF Clone, Human, untagged HG11506-UT
    Factor VII Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG14137-ACGLN
    Factor VII cDNA ORF Clone, Human, C-GFPSpark® tag HG11506-ACG
    Factor VII Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11506-ACGLN
    Factor VII cDNA ORF Clone, Human, C-GFPSpark® tag HG14137-ACG
    Factor VII cDNA ORF Clone, Human, C-OFPSpark® tag HG14137-ACR
    Factor VII cDNA ORF Clone, Human, C-OFPSpark® tag HG11506-ACR
    Factor VII cDNA ORF Clone, Human, C-DDK (Flag®) tag HG14137-CF
    Factor VII cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11506-CF
    Factor VII cDNA ORF Clone, Human, N-DDK (Flag®) tag HG14137-NF
    Factor VII cDNA ORF Clone, Human, C-His tag HG14137-CH
    Factor VII cDNA ORF Clone, Human, C-His tag HG11506-CH
    Factor VII cDNA ORF Clone, Human, N-His tag HG14137-NH
    Factor VII cDNA ORF Clone, Human, N-His tag HG11506-NH
    Factor VII cDNA ORF Clone, Human, C-HA tag HG14137-CY
    Factor VII cDNA ORF Clone, Human, C-HA tag HG11506-CY
    Factor VII cDNA ORF Clone, Human, N-HA tag HG11506-NY
    Factor VII cDNA ORF Clone, Human, N-HA tag HG14137-NY
    Factor VII cDNA ORF Clone, Human, C-Myc tag HG14137-CM
    Factor VII cDNA ORF Clone, Human, C-Myc tag HG11506-CM
    Factor VII cDNA ORF Clone, Human, N-Myc tag HG14137-NM
    Factor VII cDNA ORF Clone, Human, N-Myc tag HG11506-NM
    Factor VII qPCR Primer Pairs, Human HP101379
    info info
    添加购物车成功! 添加购物车失败!