( We provide with GFAP qPCR primers for gene expression analysis, HP101799 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 GFAP 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG12167-ACG|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG12167-ACR|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG12167-ANG|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG12167-ANR|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG12167-CF|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG12167-CH|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG12167-CM|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG12167-CY|
|人 GFAP 基因ORF全长cDNA(克隆载体)||HG12167-G|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG12167-NF|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG12167-NH|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG12167-NM|
|人 GFAP 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG12167-NY|
|人 GFAP 基因ORF全长cDNA克隆(表达载体)||HG12167-UT|
GFAP is a cell-specific marker which belongs to the intermediate filament family. It can distinguish astrocytes from other glial cells during development. GFAP is expressed in cells lacking fibronectin. It is a type III intermediate filaments protein which contains three domains: the head, rod and tail domains. GFAP functions in many important entral nervous system (CNS) processes, including cell communication and the functioning of the blood brain barrier. Improper GFAP regulation can cause multiple disorders. Defects in GFAP is related to Alexander disease which is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes.