|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10742-ACG|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10742-ACR|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10742-CF|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10742-CH|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10742-CM|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10742-CY|
|人 GP1BB/CD42c 基因ORF全长cDNA(克隆载体)||HG10742-M|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10742-M-F|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10742-NF|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10742-NH|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10742-NM|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10742-NY|
|人 GP1BB/CD42c 基因ORF全长cDNA克隆(表达载体)||HG10742-UT|
Platelet glycoprotein Ib (GPIb) complex is best known as a major platelet receptor for von Willebrand factor essential for platelet adhesion under high shear conditions found in arteries and in thrombosis. The GPIb complex is composed of GPIb alpha (Platelet glycoprotein Ib alpha chain) covalently attached to GPIb beta (Platelet glycoprotein Ib beta chain) and noncovalently complexed with GPIX and GPV. GPIb-beta, also known as GP1BB, CD42b-beta and CD42c, is single-pass type I membrane protein expressed in heart and brain, which is a critical component of the von Willebrand factor (vWF) receptor. The cysteine knot region of GPIb beta in the N terminus is critical for the conformation of GPIb beta that interacts with GPIX. The precursor of GP1BB is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. GPIb is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. GPIb alpha chain provides the vWF binding site, and GPIb beta chain contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. GP1BB is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (vWF), and mediates platelet adhesion in the arterial circulation. Defects in GP1BB are a cause of Bernard-Soulier syndrome (BSS), also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.