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人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签

    产品数据评论相关产品实验方法
     GPI cDNA Clone产品信息
    NCBI注册码:NM_024816.2
    参考序列ORF长度:1677bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens rabaptin, RAB GTPase binding effector protein 2 with C terminal Flag tag.
    分子别称:FRA, FLJ23282, RABEP2
    分子种属:Human
    载体:pCMV3-C-FLAG
    质粒:
    限制性酶切位点:
    Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with GPI qPCR primers for gene expression analysis, HP101237 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签 on other vectors
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG11352-ACG2070
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG11352-ACR2070
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG11352-ANG2070
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG11352-ANR2070
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG11352-CF1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), C-His 标签HG11352-CH1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG11352-CM1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG11352-CY1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA(克隆载体)HG11352-M920
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体)HG11352-M-N1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG11352-NF1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), N-His 标签HG11352-NH1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG11352-NM1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG11352-NY1890
    人 GPI/Glucose 6 phosphate isomerase 基因ORF全长cDNA克隆(表达载体)HG11352-UT1890
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    货号: HG11352-CF
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