|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10345-ACG|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10345-ACR|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10345-CF|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10345-CH|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10345-CM|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10345-CY|
|人 Granzyme B/GZMB 基因ORF全长cDNA(克隆载体)||HG10345-M|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10345-M-F|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10345-NF|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10345-NH|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10345-NM|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10345-NY|
|人 Granzyme B/GZMB 基因ORF全长cDNA克隆(表达载体)||HG10345-UT|
Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.