|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 HAO1 基因全长ORF克隆 (表达载体), C端GFPSpark标签||HG12076-ACG|
|人 HAO1 基因全长ORF克隆 (表达载体), C端OFPSpark标签||HG12076-ACR|
|人 HAO1 基因全长ORF克隆 (表达载体), N端GFPSpark标签||HG12076-ANG|
|人 HAO1 基因全长ORF克隆 (表达载体), N端OFPSpark标签||HG12076-ANR|
|人 HAO1 基因全长ORF克隆 (表达载体), C端FLAG标签||HG12076-CF|
|人 HAO1 基因全长ORF克隆 (表达载体), C端His标签||HG12076-CH|
|人 HAO1 基因全长ORF克隆 (表达载体), C端Myc标签||HG12076-CM|
|人 HAO1 基因全长ORF克隆 (表达载体), C端HA标签||HG12076-CY|
|人 HAO1 基因全长ORF克隆||HG12076-G|
|人 HAO1 基因全长ORF克隆 (表达载体), N端FLAG标签||HG12076-NF|
|人 HAO1 基因全长ORF克隆 (表达载体), N端His标签||HG12076-NH|
|人 HAO1 基因全长ORF克隆 (表达载体), N端Myc标签||HG12076-NM|
|人 HAO1 基因全长ORF克隆 (表达载体), N端HA标签||HG12076-NY|
|人 HAO1 基因全长ORF克隆 (表达载体), 无标签||HG12076-UT|
Hydroxyacid oxidase 1, also known as Glycolate oxidase, HAO1 and GOX1, is a member of the FMN-dependent alpha-hydroxy acid dehydrogenase family. HAO1 / GOX1 has 2-hydroxyacid oxidase activity. It is most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. HAO1 / GOX1 is a liver-specific peroxisomal enzyme that oxidizes glycolate to glyoxylate with concomitant production of H2O2. In Hao1 messenger RNA (mRNA), an iron-responsive element (IRE) homologous to the sequence recognized by iron regulatory proteins (IRP), key regulators of iron homeostasis, is present. Mammalian HAO1 / GOX1 is a peroxisomal protein and that the C-terminal sequence SKI acts as the targeting signal. Down-regulation of HAO1 / GOX1 expression during oxidative stress may provide a mechanism to prevent excessive H2O2 formation in liver peroxisomes and may represent the prototype of a poorly recognized but potentially relevant response to oxidative injury involving down-regulation of ROS-producing enzymes.