HAO1 cDNA ORF Clone, Human, C-HA tag

Cat: HG12076-CY
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All HAO1 Reagents
CRO Services
Product recommended

All HAO1 Reagents
HAO1 cDNA ORF Clone, Human, C-HA tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
1113 bp
描述
Full length Clone DNA of Human hydroxyacid oxidase (glycolate oxidase) 1 with C terminal HA tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-HA
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

HAO1 cDNA ORF Clone, Human, C-HA tag Alternative Names
GOX cDNA ORF Clone, Human;GOX1 cDNA ORF Clone, Human;HAOX1 cDNA ORF Clone, Human
HAO1 Background Information

Hydroxyacid oxidase 1, also known as Glycolate oxidase, HAO1 and GOX1, is a member of the FMN-dependent alpha-hydroxy acid dehydrogenase family. HAO1 / GOX1 has 2-hydroxyacid oxidase activity. It is most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. HAO1 / GOX1 is a liver-specific peroxisomal enzyme that oxidizes glycolate to glyoxylate with concomitant production of H2O2. In Hao1 messenger RNA (mRNA), an iron-responsive element (IRE) homologous to the sequence recognized by iron regulatory proteins (IRP), key regulators of iron homeostasis, is present. Mammalian HAO1 / GOX1 is a peroxisomal protein and that the C-terminal sequence SKI acts as the targeting signal. Down-regulation of HAO1 / GOX1 expression during oxidative stress may provide a mechanism to prevent excessive H2O2 formation in liver peroxisomes and may represent the prototype of a poorly recognized but potentially relevant response to oxidative injury involving down-regulation of ROS-producing enzymes.

Full Name
hydroxyacid oxidase (glycolate oxidase) 1
References
  • Jones J.M.et al., 2000, J. Biol. Chem. 275:12590-7.
  • Recalcati,S. et al., 2001, J Cell Sci. 114 (Pt 9):1625-9.
  • Recalcati,S. et al., 2003, Hepatology. 38 (5):1159-66.
  • Murray M.S.et al., 2008, Biochemistry 47:2439-49.
  • Bourhis J.M.et al., 2009, Acta Crystallogr. F 65:1246-53.
  • HAO1 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    HAO1 cDNA ORF Clone in Cloning Vector, Mouse MG5A0955-U
    HAO1 cDNA ORF Clone, Mouse, untagged MG5A0955-UT
    HAO1 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG5A0955-ACGLN
    HAO1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG5A0955-ACG
    HAO1 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG5A0955-ANG
    HAO1 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG5A0955-ACR
    HAO1 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG5A0955-ANR
    HAO1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG5A0955-CF
    HAO1 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG5A0955-NF
    HAO1 cDNA ORF Clone, Mouse, C-His tag MG5A0955-CH
    HAO1 cDNA ORF Clone, Mouse, N-His tag MG5A0955-NH
    HAO1 cDNA ORF Clone, Mouse, C-HA tag MG5A0955-CY
    HAO1 cDNA ORF Clone, Mouse, N-HA tag MG5A0955-NY
    HAO1 cDNA ORF Clone, Mouse, C-Myc tag MG5A0955-CM
    HAO1 cDNA ORF Clone, Mouse, N-Myc tag MG5A0955-NM

    HAO1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    HAO1 cDNA ORF Clone in Cloning Vector, Human HG12076-G
    HAO1 cDNA ORF Clone, Human, untagged HG12076-UT
    HAO1 cDNA ORF Clone, Human, C-GFPSpark® tag HG12076-ACG
    HAO1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG12076-ACGLN
    HAO1 cDNA ORF Clone, Human, N-GFPSpark® tag HG12076-ANG
    HAO1 cDNA ORF Clone, Human, C-OFPSpark® tag HG12076-ACR
    HAO1 cDNA ORF Clone, Human, N-OFPSpark® tag HG12076-ANR
    HAO1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12076-CF
    HAO1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG12076-NF
    HAO1 cDNA ORF Clone, Human, C-His tag HG12076-CH
    HAO1 cDNA ORF Clone, Human, N-His tag HG12076-NH
    HAO1 cDNA ORF Clone, Human, N-HA tag HG12076-NY
    HAO1 cDNA ORF Clone, Human, C-Myc tag HG12076-CM
    HAO1 cDNA ORF Clone, Human, N-Myc tag HG12076-NM
    HAO1 qPCR Primer Pairs, Human HP101604
    info info
    添加购物车成功! 添加购物车失败!