快速订购

人 HDAC1 基因ORF全长cDNA克隆(表达载体), C-HA 标签

  • Human HDAC1 natural ORF mammalian expression plasmid, C-HA tag
产品数据评论相关产品实验方法
 HDAC1 cDNA Clone产品信息
NCBI注册码:NM_004964.2
参考序列ORF长度:1449bp
cDNA基因描述:Full length Clone DNA of Homo sapiens histone deacetylase 1 with C terminal HA tag.
分子别称:HD1, RPD3, GON-10, RPD3L1, DKFZp686H12203, HDAC1
分子种属:Human
载体:pCMV3-C-HA
质粒:pCMV3-HDAC1-HA
限制性酶切位点:KpnI + XbaI (6kb + 1.49kb)
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with HDAC1 qPCR primers for gene expression analysis, HP101362 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 HDAC1 基因质粒图
Human HDAC1 natural ORF mammalian expression plasmid, C-HA tag
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Product nameProduct name
研究背景

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. Histone Deacetylase 1 protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

参考资料
  • Beharry AW, Sandesara PB, Roberts BM, Ferreira LF, Senf SM, Judge AR. HDAC1 activates FoxO and is both sufficient and required for skeletal muscle atrophy. Journal of Cell Science. 2014;127(7):1441-1453.
  • Size / Price
    货号: HG11486-CY
    目录价: 
    单价:      (You Save: )

    Datasheet & Documentation

    Contact Us
      您曾浏览过的产品
        产品数据可能随批次变化,最新数据请详见随货说明书。
        请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”