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人 HEXA 基因ORF全长cDNA克隆(表达载体), C-His 标签

  • Cynomolgus CD19/B4/CVID3 Gene Plasmid Map 5620
产品数据评论相关产品实验方法
 HEXA cDNA Clone产品信息
NCBI注册码:NM_000520
参考序列ORF长度:1635 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens hexosaminidase A (alpha polypeptide) with C terminal His tag.
分子别称:HEXA
分子种属:Human
载体:pCMV3-C-His
质粒:pCMV3-HEXA-His
限制性酶切位点:HindIII + XbaI(6kb+1.64kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1518A/G not causing the amino acid variation.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with HEXA qPCR primers for gene expression analysis, HP101571 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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参考资料
  • Norflus,F. et al., 1996, DNA Cell Biol. 15 (2):89-97.
  • Kaufman M., et al., 1997, Hum. Mutat. 10:295-300.
  • Tanaka A., et al., 2003, J. Hum. Genet. 48:571-574.
  • Chen R., et al., 2009, J. Proteome Res. 8:651-661.
  • Burkard T.R., et al., 2011, BMC Syst. Biol. 5:17-17.
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    货号: HG12037-CH
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