c-MET cDNA ORF Clone, Human, C-DDK (Flag®) tag

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c-MET cDNA ORF Clone, Human, C-DDK (Flag®) tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
4173 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human met proto-oncogene (hepatocyte growth factor receptor) transcript variant 2 with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + NotI (6kb + 4.21kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

c-MET cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

c-MET cDNA ORF Clone, Human, C-DDK (Flag®) tag: 验证图片

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

c-MET cDNA ORF Clone, Human, C-DDK (Flag®) tag: 别称

AUTS9 cDNA ORF Clone, Human; c-Met cDNA ORF Clone, Human; DFNB97 cDNA ORF Clone, Human; HGFR cDNA ORF Clone, Human; RCCP2 cDNA ORF Clone, Human

c-MET 背景信息

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
全称
MET proto-oncogene, receptor tyrosine kinase
参考文献
  • McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
  • Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
  • Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
  • Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
  • Triptolide-assisted phosphorylation of p53 suppresses inflammation-induced NF-κB survival pathways in cancer cells
    Author
    Zheng, L;Jia, J;Dai, H;Wan, L;Liu, J;Hu, L;Zhou, M;Qiu, M;Chen, X;Chang, L;Kim, JY;Reckamp, K;Raz, DJ;Xia, Z;Shen, B;
    Year
    2017
    Journal
    Mol. Cell. Biol.
    Application
    Control
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