Human HS3ST1 Baculovirus-Insect Overexpression Lysate

Cat: 11810-H07BL

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Human HS3ST1 Baculovirus-Insect Overexpression Lysate 产品信息

Product Description
This Human HS3ST1 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of HS3ST1 protein (Cat: 11810-H07B) from the overexpression lysate was verified.
Expression Host
Baculovirus-Insect Cells
Sequence Information
A DNA sequence encoding the human HS3ST1 (AAH57803.1) (Arg21-His307) was expressed with a polyhistidine tag at the N-terminus.
Molecule Mass
The recombinant human HS3ST1 consists 303 amino acids and predicts a molecular mass of 36 kDa.

Human HS3ST1 Baculovirus-Insect Overexpression Lysate Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human HS3ST1 Baculovirus-Insect Overexpression Lysate Alternative Names

Human 3OST Overexpression Lysate;Human 3OST1 Overexpression Lysate

HS3ST1 Background Information

Full Name
heparan sulfate (glucosamine) 3-O-sulfotransferase 1
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